Gold nanoparticle-based competitive colorimetric assay for detection of protein–protein interactions

Charng-Sheng Tsai, Ting-Bin Yu, Chao-Tsen Chen
2005 Chemical Communications  
A gold nanoparticle-based competitive colorimetric assay uses the ensemble of Concanavalin (ConA) and mannopyranosideencapsulated gold nanoparticles (Man-GNPs) to identify the binding partners for ConA and the binding constants are determined based on the wavelength shifts. ; Fax: 886 2 23636359; Tel: 886 2 23645819 { Electronic supplementary information (ESI) available: Synthetic procedures for mannopyranoside, UV-vis spectra of Man-GNPs in the presence of individual tested protein,
more » ... protein, MALDI-TOF-MS spectra, Job's plot, Hill plot for stoichiometry determination and the curve fitting for the binding constant. See http://dx.
doi:10.1039/b507237a pmid:16113719 fatcat:7pdyvwwnnncx5n5vtt6z7z2doa