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Proteins of Insoluble Matrix of Avian (Gallus Gallus) Eggshell
Connective Tissue Research
The protein composition of the insoluble avian eggshell matrix was studied. The determination of these proteins insoluble in water (EDTA-insoluble) was carried out using enzymatic cleavage followed by a high-performance liquid chromatography-mass spectrometry method. The influence of various enzymes on the protein splitting also was studied. The distribution of proteins depends on the type of layer (localization within the eggshell): ovocalyxin-32 was found mainly in the outer layer (thedoi:10.1080/03008200601003116 pmid:17364661 fatcat:qjsyhzszzngwdacgjd522unn2q