Internet Archive Scholar

System-level studies of a cell-free transcription-translation platform for metabolic engineering [article]

Yong Y. Wu, Hirokazu Sato, Hongjun Huang, Stephanie J. Culler, Julia Khandurina, Harish Nagarajan, Tae Hoon Yang, Stephen Van Dien, Richard M. Murray
2017 biorxiv/medrxiv   pre-print

Preserved Fulltext

fulltext thumbnail
Web Archive Capture PDF (1.6 MB)
https://web.archive.org/web/20190427021905/https://authors.library.caltech.edu/86111/1/172007.full.pdf

A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL. The file type is application/pdf.

Current methods for assembling biosynthetic pathways in microorganisms require a process of repeated trial and error and have long design-build-test cycles. We describe the use of a cell-free transcription-translation (TX-TL) system as a biomolecular breadboard for the rapid engineering of the 1,4-butanediol (BDO) pathway. We demonstrate the reliability of TX-TL as a platform for engineering biological systems by undertaking a careful characterization of its transcription and translation
more » ... ities and provide a detailed analysis of its metabolic output. Using TX-TL to survey the design space of the BDO pathway enables rapid tuning of pathway enzyme expression levels for improved product yield. Leveraging TX-TL to screen enzyme variants for improved catalytic activity accelerates design iterations that can be directly applied to in vivo strain development.
doi:10.1101/172007 fatcat:blyapxxvbfbe5jjciz3qrzw2ce
Citation

Yong Y. Wu, Hirokazu Sato, Hongjun Huang, Stephanie J. Culler, Julia Khandurina, Harish Nagarajan, Tae Hoon Yang, Stephen Van Dien, Richard M. Murray. "System-level studies of a cell-free transcription-translation platform for metabolic engineering." biorxiv/medrxiv (2017)