Identification and characterisation of vesicles in resorbing osteoclasts using electron tomography

Emma McDermott, David Sunderhauf, Kevin Mackenzie, Debbie Wilkinson, Miep Helfrich
2016 Bone Abstracts  
Methodology 1. Osteoclasts were isolated from rabbit limbs and cultured on dentine discs. 2. The discs were processed for routine TEM and embedded in resin. 3. 200-300 nm thick sections were placed onto coated Cu grids. 4. Grids were placed in TEM for tilt series (±65) image acquisition. 5. IMOD software-tomogram generated. 6. Amira software -3D renderings generated.
doi:10.1530/boneabs.5.p190 fatcat:jeausacj7vbqfehu3bo4h4syga