THU0097 The discrepancy between mrna and protein expression of the tumour-suppressor gene maspin in synovial tissue might contribute to synovial hyperplasia in rheumatoid arthritis (ra)
Speaker abstracts 2001
immunocompetence by assessing function of selected cell types involved in host resistance. Methods Basal NK cell activity, lymphoproliferation following stimulation with anti-CD3, pokeweedmitogen or concanavalin A, and antibody forming cell response (AFC) were determined in splenocytes from female rats (F-344; N = 12 per group) treated with placebo, IL-1ra alone (20 or 200 mg/kg, SC daily), sTNF-RI alone (5 or 50 mg/kg, SC twice weekly) or the dose combinations of the two cytokine inhibitors
... 21 days. Satellite groups received positive control agents for the NK cell assay (0.5 mL anti-asialo GM 1 antibody) or the mitogenesis and AFC assay (25 mg/kg cyclophosphamide). Results Basal NK cell response was increased 21% (p < 0.05) over placebo control only at the combination of 200 mg/kg IL-1ra + 5 mg/kg sTNF-RI. No effect was noted with IL-1ra or TNF-RI alone or at higher combination doses. Cells from IL-1ra (200 mg/kg) treated animals showed minimal decreases (30%, p < 0.05) on lymphocyte proliferation only in response to anti CD3-mediated proliferation. Statistically significant differences (+10 to -48%) in proliferative activity to all mitogens were observed with sTNF-RI alone or in combination with IL1-ra, but were not dose related. Diminution in the AFC response (29-36%) was noted for sTNF-RI alone (50 mg/kg) or in combination with both Il-1ra doses compared to the control group; however the differences were not statistically significant. IL1-ra alone, the low dose of sTNF-RI (5 mg/kg) alone or in combination with IL-1ra doses had no significant effect on AFC response. Conclusion Thus, IL-1ra alone has minimal effects on selected functions of the immune system. Effects on immune function noted at 50 mg/kg sTNF-RI were considered minimal and importantly were not exacerbated by the combination of IL-1ra.