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A Simple Approach for Counting CD4+ T Cells Based on a Combination of Magnetic Activated Cell Sorting and Automated Cell Counting Methods
Frequent tests for CD4+ T cell counting are important for the treatment of patients with immune deficiency; however, the routinely used fluorescence-activated cell-sorting (FACS) gold standard is costly and the equipment is only available in central hospitals. In this study, we developed an alternative simple approach (shortly named as the MACS-Countess system) for CD4+ T cell counting by coupling magnetic activated cell sorting (MACS) to separate CD4+ T cells from blood, followed by countingdoi:10.3390/app11219786 fatcat:5ez55hlhabdmtodvxzdsocj5hq