The Importance of Standardization on Analyzing Circulating RNA

Inyoul Lee, David Baxter, Min Young Lee, Kelsey Scherler, Kai Wang
2016 Molecular Diagnosis & Therapy  
Circulating RNAs, especially microRNAs (miRNAs) have recently emerged as non-invasive disease biomarkers. Despite enthusiasm and numerous reports on disease associated circulating miRNAs, currently there is no circulating miRNA-based diagnostic in use. In addition, there are many contradictory reports on the concentration changes of specific miRNA in circulation. Here we review the impact of various technical and non-technical factors related to circulating miRNA measurement and elucidate the
more » ... portance of having a general guideline for sample preparation and concentration measurement in studying circulating RNA.
doi:10.1007/s40291-016-0251-y pmid:28039578 pmcid:PMC5426982 fatcat:56s42d6l6narze4mp6lhnxubda