Improvement of avian leukosis virus (ALV)-based retrovirus vectors by using different cis-acting sequences from ALVs

F L Cosset, C Legras, J L Thomas, R M Molina, Y Chebloune, C Faure, V M Nigon, G Verdier
1991 Journal of Virology  
Production and expression of double-expression vectors which transduce both Neor and lacZ genes and are based on the structure of avian leukosis virus were enhanced by using cis-acting sequences (long terminal repeats and noncoding sequences) from Rous-associated virus-1 and Rous-associated virus-2 rather than those of avian erythroblastosis virus previously used in our constructs. Polyclonal producer cells obtained after transfection of these vectors into the Isolde packaging cell line gave
more » ... g cell line gave rise to titers as high as 3 x 105 lacZ CFU/ml, whereas it was possible to isolate clones of producer cells giving rise to titers of more than 106 resistance focus-forming units per ml.
doi:10.1128/jvi.65.6.3388-3394.1991 fatcat:xw5gn2bqfjcerl3u34sv7kdbra