EXPRESSION OF THE INTERFERON RESPONSIVE GENE IN THE COMMON CARP (CYPRINUS CARPIO)
Egyptian Journal of Aquatic Biology and Fisheries
iral haemorrhagic septicaemia (VHS) is an important rhabdo viral disease in the fish farming . Over recent years a highly effective experimental DNA vaccine to VHS has been developed. Virus-induced genes were ultimately retrieved from the subtracted cDNA library, and their differential expression was further confirmed by semi quantitative reverse transcription-PCR and Northern blot analysis. The ability of the plasmid to mediate expression of the viral G-protein on the surface of transfected
... e of transfected cells was confirmed by specific immunostaining for the viral protein. The type 1 interferon (IFN) system is a rapid and powerful antiviral defense. Using a luciferase cell line to detect expression of IFN, it was shown that the Common Carp (Cyprinus carpio) Gonad cell line (CCG -p1), produces IFN when transfected with a plasmid encoding the glycoprotein of VHSV, but not with plasmid vector alone. The results showed that the cells transfected with pcDNA3 vhs-G plasmid were expressing G protein, while the cells transfected with pcDNA3 plasmid did not show any staining. The cells transfected with the pcDNA3 vhs-G showed an increased luciferase activity of 5.4-fold compared with the control non-transfected cells . this increase was statistically significant (p < 0.05).