The fossil energy sources used in the world are gradually decreasing and limited. Fossil fuels cause environmental pollution, and the unit price is constantly increasing. For this reason, demand for cheaper and renewable energy sources that do not pollute the environment is increasing day by day. The sweet sorghum plant has attracted attention in recent years with its high biomass yield, sugar content and bioethanol yield. In this study, it was aimed to determine the bio-fuel potential of some

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... weet sorghum genotypes in semi-arid climatic conditions. The experiment was set up in randomized complete block design with 4 replicates. Research was carried out in 2015 under Harran Plain (36o 42' N and 38o 58' E) second crop conditions, Sanliurfa, Turkey. In the study 49 genotypes of sweet sorghum were used. Stalk yield, juice yield, syrup yield, brix, sugar yield and theoretical ethanol yield were determined in the study. Significant differences were found between the genotypes for tested characteristics (P≤0.01). Stalk yield ranged from 7110.0 kg da-1 to 24262.5 da-1, juice yield from 2550.0 L da-1 to 12187.5 L da-1, syrup yield from 291.4 L da-1 to 2242.5 L da-1. Also, brix value varied between %7.0 and %18.87, sugar yield between 247.7 da-1 and 1906.1 da-1, Theoretical ethanol yield between 131.9 L da-1 and 1014.8 L da-1. Considering to stalk yield, juice and syrup yield, brix, sugar yield and theoretical ethanol yield; Nebraska sugar, Topper 76, Smith, M81E and Corina genotypes were found as the best. As a result of research, 21 genotypes with better performance than the others were selected for further studies.