Altered expression of cyclophilin B (CyPB) in chemically induced hepatocarcinogenesis of rat, which was subjected to the Solt-Farber protocol, was analyzed by anti-N-terminal peptide antibody for CyPB. In a group of the partially hepatectomized rats after administration of diethylnitorsamine (DEN) interperitoneally followed by the oral administration of 2-acethylaminofluorene (FAA) , marked induction of a tumor-specific marker GST-P and the formation of the precancerous lesion in rat liver were

more »

... observed. A band of approximately 20 kDa molecular weight was detected by anti-N-terminal peptide antibody in the normal liver and testis. This band was estimated as a CyPB protein.The levels of CyPB in the precancerous lesion were increased from 1 week after hepatectomy, and the increase was approximately 2-fold up to 6 weeks. In addition, the levels of CyPB in serum were gradually increased from 4 to 7 week with delayed kinetics in the liver. The levels of CyPB were increased approximately 1.6-fold at the point of 7 week after the hepatectomy. These results suggested the possible role of CyPB as a tumor marker of the hepatocellular carcinoma.