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Cell Chemical Biology
RNA-modifying enzymes are difficult to assay due to the absence of fluorometric substrates. Here we show that the Broccoli, a previously reported fluorescent RNA-dye complex, can be modified to contain N 6 -methyladenosine, a prevalent mRNA base modification. Methylated Broccoli is nonfluorescent, but upon demethylation by the RNA demethylases Fat mass and obesityassociated protein (FTO) or ALKBH5 binds and activates the fluorescence of its cognate fluorophore. We describe a high-throughputdoi:10.1016/j.chembiol.2015.11.018 pmid:26877022 pmcid:PMC4800036 fatcat:sxiiafa4xjhg7e4iuedklgtwra