The current study was conducted for microbial analysis, viable cell count and antibiotics sensitivity test of bacterial isolates from Anbar and Kernel Sher Kali streams water. Two samples were collected from two different areas of Anbar and Kernel Sher Kali streams water. These samples were screened for bacteriological and fungal study and antibiotics susceptibility testing for isolates. The water samples were analyzed for bacteriological study by Multiple Fermentation Tubes Methods and PDA

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... a growth for fungal spores. The isolated bacteria were confirmed on the basis of indole test, citrate test, catalase test and urease test.The result of the study showed that Escherichia Coli, Klebsiella oxytoca, Klebsiella pneumonia, Candida andpencillium chrysogenum were present inboth samples. Most probable numberfor Anbar stream water sample was 15 per 100ml with 95% confidence low rate was 4.5 and high rate was 42 while MPN for kernel Sher kali stream water sample was 27 with 95% confidence low rate was 8.7 and high confidence rate was 94.According to Antibiotic susceptibility profile E. coli and Klebsiella oxytoca from Anbar sample showed resistance to Amoxicillin, Ampicillin, Penicillin G, teicoplanin whereas Klebsiella oxytocaalso found resistant to Fosfamycin. Bacterial strains i.e. Klebsiella pneumonia and E.coli isolated from water stream of Kernel Sher Kali were found resistant to Ampicillin, Penicillin G, teicoplanin while. Klebsiella pneumonia was found resistant to Amoxicillin. CFU for the Anbar stream water were ranging from 8.8×10 3 to 4.2×10 5 and for the Kernel Sher Kali stream water were ranging from 1.1×10 4 to 3.6×10 5 . Both samples contain coliform contamination and does not meet the WHO standard and unfit to use for swimming or house hold activities.