Ryegrasses are important forage species. An important foliar disease in Lolium is crown rust, caused by the fungus Puccinia<br />coronata. This cosmopolitan biotrophic fungus causes forage and seed losses, and decoloration of amenity grasslands.<br />In breeding towards resistant cultivars, it is important to know the mode of inheritance of crown rust resistance. A pair<br />cross between a susceptible and a resistant plant resulted in a F1 population segregating for rust resistance. According

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... o<br />the segregation data, major genes were assumed to encode for crown rust resistance. In a bulk segregant analysis (BSA)<br />187 primer combinations revealed AFLP markers linked with resistance. Two groups of genetically unlinked markers<br />were identified, explaining together 35% of the phenotypic variation.