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Pulsed-field gel electrophoresis was used to analyze the genomic organization of 16 bacteria belonging or related to the familyRhizobiaceae of the alpha subgroup of the classProteobacteria. The number and sizes of replicons were determined by separating nondigested DNA. Hybridization of anrrn gene probe was used to distinguish between chromosomes and plasmids. Members of the genus Agrobacterium all possess two chromosomes, and each biovar has a specific genome size. As previously demonstrateddoi:10.1128/jb.180.10.2749-2755.1998 fatcat:vybpp73x4zfohfeb7dhw65jmxa