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The intracellular mechanism that mediates the stimulatory effect of prostaglandin F2α (PGF2α) on progesterone secretion by bovine luteal cells is not well understood. In the present study, we investigated to test the hypothesis that PGF2α stimulates progesterone secretion through activation of protein kinase C (PKC) by using a cell culture system. Bovine mid-luteal cells (Days 8-12 of the estrous cycle) were cultured for 24 h and then exposed to varying concentrations of PGF2α (10 -8 , 10 -7 ,doi:10.1262/jrd.44.79 fatcat:zgznahsokbecplwjzx6qbv2iyy