Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy

Seungrag Lee, Young Ran Kim, Ji Yong Lee, Joon Haeng Rhee, Chang-Soo Park, Dug Young Kim
2011 Journal of Biomedical Optics  
We present the real-time quantitative analysis of Vibrio vulnificus-infected host cells using quantitative phase microscopy (QPM) based on interferometric techniques. This provides the ability to retrieve the phase or optical path-length distribution over the cell with nanometer path-length sensitivity from a single interferogram image. We have used QPM to study dynamic cell morphologic changes and to noninvasively quantify the cell volumes of rat basophilic leukemia RBL-2H3 cells infected with
more » ... cells infected with V. vulnificus strains: wild type (MO6-24/O) and RtxA1 toxin mutant (CMM770). During the process of V. vulnificus infection in RBL-2H3 cells, the dynamic changes of quantitative phase images, cell volumes, and areas were observed in real time using QPM. In contrast, dramatic changes were not detected in RBL-2H3 cells infected with the noncytotoxic RtxA1 toxin mutant. The results showed good correlation between QPM analysis and biochemical assays, such as lactate dehydrogenase assay or β-hexosaminidase release assay. We suggest that QPM is a powerful quantitative method to study the dynamic process of host cells infected with pathogens in a noninvasive manner. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).
doi:10.1117/1.3548882 pmid:21456868 fatcat:aldhbdj6qnbspoukqw75kumvfu