A power-efficient thermocycler based on induction heating for DNA amplification by polymerase chain reaction

Debjani Pal, V. Venkataraman, K. Naga Mohan, H. Sharat Chandra, Vasant Natarajan
2004 Review of Scientific Instruments  
We have built a thermocycler based on the principles of induction heating for polymerase chain reaction (PCR) of target sequences in DNA samples of interest. The cycler has an average heating rate of ϳ0.8°C/s and a cooling rate of ϳ0.5°C/s, and typically takes ϳ4 h to complete a 40-cycle PCR protocol. It is power-efficient (ϳ6 W per reaction tube), micro-processor controlled, and can be adapted for battery operation. Using this instrument, we have successfully amplified a 350 bp segment from a
more » ... bp segment from a plasmid and SRY, the human sex determining gene, which occurs as a single-copy sequence in genomic DNA of human males. The PCR products from this thermocycler are comparable to those obtained by the use of commercially available machines. Its easy front-end operation, low-power design, portability and low cost makes it suitable for diagnostic field applications of PCR.
doi:10.1063/1.1781368 fatcat:x43ypg2bh5ehffvmtkhzamm4aq