Calcium negatively regulates secretion from dense granules inToxoplasma gondii [article]

Nicholas J Katris, Geoff I McFadden, Giel G van Dooren, Ross F Waller
2018 bioRxiv   pre-print
Apicomplexan parasites includingToxoplasma gondiiandPlasmodiumspp. manufacture a complex arsenal of secreted proteins used to interact with and manipulate their host environment. These proteins are organised into three principle exocytotic compartment types according to their functions: micronemes for extracellular attachment and motility, rhoptries for host cell penetration, and dense granules for subsequent manipulation of the host intracellular environment. The order and timing of these
more » ... s during the parasite's invasion cycle dictates when exocytosis from each compartment occurs. Tight control of compartment secretion is, therefore, an integral part of apicomplexan biology. Control of microneme exocytosis is best understood, where cytosolic intermediate molecular messengers cGMP and Ca2+act as positive signals. The mechanisms for controlling secretion from rhoptries and dense granules, however, are virtually unknown. Here, we present evidence that dense granule exocytosis is negatively regulated by cytosolic Ca2+, and we show that this Ca2+-mediated response is contingent on the function of calcium-dependent protein kinasesTgCDPK1 andTgCDPK3. Reciprocal control of micronemes and dense granules provides an elegant solution to the mutually exclusive functions of these exocytotic compartments in parasite invasion cycles and further demonstrates the central role that Ca2+signalling plays in the invasion biology of apicomplexan parasites.
doi:10.1101/386722 fatcat:u5kcfenurfhoxgxrcr2x7ajesq