Determination of Aflatoxin B 1 in Highly Contaminated Peanut Samples Using HPLC and ELISA

Ramón Asis, Romina D. Di Paola, Mario A. J. Aldao
2002 Food and Agricultural Immunology  
Regulatory authorities, the food industry and the consumer demand reliable determination of chemical contaminants present in foods. A relatively new analytical technique that addresses this need is an immunobiosensor based on surface plasmon resonance (SPR) measurements. Although a range of tests have been developed to measure residues in milk, meat, animal bile and honey, a considerable problem has been encountered with both serum and plasma samples. The high degree of non-specific binding of
more » ... ome sample components can lead to loss of assay robustness, increased rates of false positives and general loss of assay sensitivity. In this paper we describe a straightforward precipitation technique to remove interfering substances from serum samples to be analysed for veterinary anthelmintics by SPR. This technique enabled development of an assay to detect a wide range of benzimidazole residues in serum samples by immunobiosensor. The limit of quantification was below 5 ng/ ml and coefficients of variation were about 2%.
doi:10.1080/09540100220145000 fatcat:pdlmg2qvzzgxdbgc3h4fgtyasi