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Analysis of rev gene function on human immunodeficiency virus type 1 replication in lymphoid cells by using a quantitative polymerase chain reaction method
Journal of Virology
Most detailed analyses of the human immunodeficiency virus type 1 (HIV-1) rev gene product have relied on transfection of subgenomic env constructs into cells in which amplification of the transfected DNA occurs. This was necessitated by difficulties in quantitating low-abundance HIV-1 mRNA species and in distinguishing different RNAs of similar sizes. We have modffied the conventional polymerase chain reaction method for general use as an extremely sensitive procedure for quantitative analysisdoi:10.1128/jvi.63.11.4875-4881.1989 fatcat:cwv2w7mh2ngvpbmz7q4fjf5s54