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Protein Misfolding and Inclusion Body Formation in RecombinantEscherichia coliCells Overexpressing Heat-shock Proteins
Journal of Biological Chemistry
PreS2-S-␤-galactosidase, a three-domain fusion protein that aggregates extensively in the cytoplasm of Escherichia coli, was used to systematically investigate the effects of heat-shock protein (hsp) overproduction on protein misfolding and inclusion body formation. While the co-overexpression of the DnaK and DnaJ molecular chaperones led to a 3-6-fold increase in the recovery of enzymatically active preS2-S-␤-galactosidase over a wide range of growth temperatures (30 -42°C), an increase in thedoi:10.1074/jbc.271.19.11141 pmid:8626659 fatcat:yu6ycshkdna53i4ghh2eldo6qa