Tarbell, John M., Lucas Demaio, and Mark M. Zaw. Effect of pressure on hydraulic conductivity of endothelial monolayers: role of endothelial cleft shear stress. J. Appl. Physiol. 87(1): 261-268, 1999.-Significant changes in transvascular pressure occur in pulmonary hypertension, microgravity, and many other physiological and pathophysiological circumstances. Using bovine aortic endothelial cells grown on porous, rigid supports, we demonstrate that step changes in transmural pressure of 10, 20,

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... nd 30 cmH 2 O induce significant elevations in endothelial hydraulic conductivity (L p ) that require 5 h to reach new steady-state levels. The increases in L p can be reversed by addition of a stable cAMP analog (dibutyryl cAMP), and the increases in L p in response to pressure can be inhibited significantly with nitric oxide synthase inhibitors (N G -monomethyl-L-arginine and nitro-Larginine methyl ester). The increase in L p was not due to pressure-induced stretch because the endothelial cell (EC) support was rigid. It is unlikely that the increase in L p was due to a direct effect of pressure because exposure of the cells to elevated pressure (25 cmH 2 O) for 4 h had no effect on the volume flux driven by a transmural pressure of 10 cmH 2 O. We hypothesize that elevated endothelial cleft shear stress induced by elevated transmural flow in response to elevated pressure stimulates the increase in L p through a nitric oxide-cAMP-dependent mechanism. This is consistent with recent studies of the effects of shear stress on the luminal surface of ECs. We provide simple estimates of endothelial cleft shear stress, which suggest magnitudes comparable to those imposed by blood flow on the luminal surface of ECs. endothelial cells; transmural pressure; nitric oxide; adenosine 3Ј,5Ј-cyclic monophosphate