GUIDES: sgRNA design for loss-of-function screens [article]

Joshua A. Meier, Feng Zhang, Neville Sanjana
2017 bioRxiv   pre-print
GUIDES (Graphical User Interface for DNA Editing Screens) is a web-based tool for the design of custom, large-scale CRISPR libraries for loss-of-function screens in human and mouse. GUIDES combines multi-tissue RNA-sequencing data to target expressed exons, protein annotation to target functional domains, sophisticated on-target and off-target guide RNA scoring and other optimizations to create CRISPR libraries directly from a list of genes without requiring any programming expertise.
doi:10.1101/168849 fatcat:upwnmvrljfacbmrtxfjpkjsmje