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Enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between Brucella melitensis-infected and B. melitensis Rev.1-vaccinated sheep
Clinical and Diagnostic Laboratory Immunology
The problem of differentiating sheep infected with Brucella melitensis from those vaccinated or exposed to cross-reacting organisms has not been resolved by conventional serological tests or through the use of the smooth lipopolysaccharide in primary binding assays. We therefore analyzed sera from ewes experimentally infected with B. melitensis H38, from ewes naturally infected with B. melitensis, and from B. melitensis Rev.1vaccinated ewes by enzyme-linked immunosorbent assay with threedoi:10.1128/cdli.3.3.305-308.1996 fatcat:fis2moazn5byvo72hairgsk46e