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Highlights from Recent Cancer Literature
Weber and colleagues use the CRISPR/Cas9 system to multiplex functional genomic analyses. In models of intrahepatic cholangiocarcinoma and hepatocellular carcinoma, the authors perform an in vivo screen targeting a panel of tumor suppressor genes. Plasmid delivery to hepatocytes via hydrodynamic tail vein injection enabled in vivo screening of 10 single guide RNA sequences (sgRNA) in combination with oncogenic Kras G12D and 18 sgRNAs in combination with CCl 4 treatment. High mutant readdoi:10.1158/0008-54220.127.116.11 fatcat:cgwsqi3zkjbzbcfiwqw35fifti