The p16INK4aAntibody Immobilization Method for Immonosensor Application

Li Yang, Xian-He Huang, Liang Sun
2015 Asian Pacific Journal of Cancer Prevention  
The p16 INK4a is a protein that expressed in Liquid-based cervical cytology specimens and has been proved link to cervical cancer. The p16 INK4a could be detection by piezoelectric immunosensor and the immobilization of the p16 INK4a antibody influence the sensitivity of the piezoelectric immunosensor. Materials and Methods: 5μL mouse polyclonal antibody against p16 INK4a was bound onto the surface of immonosensor through two methods. (directly immobilized method; protein A method). Absorb of
more » ... e p16 INK4a antibody on the surface of immonosensor caused a shift in the resonant frequency of the immunosensor and The frequency changes recorded showed a better reproducibility. The activity of the immobilization antibody with the directly method and protein A method was tested with p16 INK4a antigen. Results: The resonant frequency for different antibody immobilization methods were different, and the sensitivity for p16 INK4a detection also different. Conclusions: The protein A method was found to be much more better than the directly method for the immobilization of the p16INK4A antibody on the gold electrode of the quartz crystal for cervical lesion detection. The Protein A method created more reproducible and stable immobilization antibody layers with p16INK4A antigen.
doi:10.7314/apjcp.2015.16.12.5115 fatcat:fdd2bei2hjhmpm57q7uedmahsu