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Expression and Immunogenicity of the Spike Glycoprotein of Porcine Respiratory Coronavirus Encoded in the E3 Region of Adenovirus
[chapter]
1995
Advances in Experimental Medicine and Biology
The full length spike (S) gene of porcine respiratory coronavirus (PRCV) was inserted into the genome of human adenovirus type 5 downstream of the early transcription region 3 promoter. The recombinant virus replicated in cultures of the swine testicle ST cell line and directed the synthesis of S antigen to an amount of approximately 33 J.lg per 10 6 cells, as determined by ELISA. The antigen was cell-associated except in the late phase of the infection, when a low amount (4 J.lg per 10 6
doi:10.1007/978-1-4615-1899-0_42
fatcat:srwumdtqh5ep5imbxnexlweqle