Manual Liquid Based Cytology in Breast Fine Needle Aspiration – Comparison with the Conventional Smear

Dr. Karan Mahinderu, Dr. Nandini N M, Arnaw Kishore, Dr. Anil Kumar Singh
2016 IOSR Journal of Dental and Medical Sciences  
Breast carcinoma is the malignant tumour that causes highest mortality among women worldwide. Liquid based smears are superior to conventional smears (CS) with regard to monolayer cell preparation, cell preservation and clear background. Liquid based cytology (LBC) has gained acceptance for use in prospective studies, because of its usefulness in immunocytochemistry (ICC) and molecular biology. Objectives: To study the performance of Manual liquid based cytology (MLBC) of neoplastic breast
more » ... plastic breast lesions in comparison to conventional fine needle aspiration (FNA) smears and to correlate the findings with Histopathology wherever possible. Results/observations: Smears for MLBC and conventional FNA were prepared from the samples from 100 female patients in the age group of 15-70 years. Simultaneously unstained smears were also prepared to be utilized for ICC. Sensitivity and specificity for both MLBC and CS was 95.2% and 100% respectively. Positive predictive value and negative predictive value for MLBC and CS was 100% and 96.9% respectively. Estrogen receptor analysis performed on five unstained MLBC smears as well on biopsy sections of the same five cases were reported as negative. Conclusion: Liquid based cytology can be safely used as an important adjunct to conventional FNA. Liquid based cytology ensures better cellular preservation, less cell overlapping and elimination of blood and excessive inflammation compared to CS. Manual liquid cytology is a technique which can be as effective a diagnostic tool as CS in low-resource settings like India. Utilization for ancillary tests such as ICC for hormonal receptors and molecular biology is an additional advantage of MLBC.
doi:10.9790/0853-150771724 fatcat:wfyqpuay7zcb7mzfutdbaml4aa