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Measurement of the sperm binding capacity of the mouse zona pellucida and its use in the estimation of anti-zona antibody titres
Conditions have been defined under which the sperm binding capacity of the mouse zona pellucida can be measured. As a measure of the concentration of anti-zona antibodies the inhibition of sperm binding appears to be both repeatable and sensitive. Using this technique anti-zona antibody titres were monitored in 6 rats actively immunized with extracts of mouse ovarian tissue. These animals exhibited a rapid rise in anti-zona antibody titre following the induction of immunity, and an associateddoi:10.1530/jrf.0.0630295 pmid:7028984 fatcat:byuubkyz7rbjvlyiovgvaglxhq