Epstein-Barr virus DNA associated with gastric adenocarcinoma and adjacent non-cancerous mucosa in patients from Manaus, Brazil

P.F. de Aquino, P.C. Carvalho, J.S. da Gama Fischer, A.Q.L. de Souza, J.S. Viana, S.R.S. Chalub, A.D.L. de Souza, M.G.C. Carvalho
2012 Genetics and Molecular Research  
Gastric cancer is one of most frequent causes of death in Brazil. The city of Manaus has one of the highest incidences of this disease in Brazil. The Epstein-Barr virus (EBV) is a ubiquitous herpesvirus that is classified as a group 1 carcinogen by the International Agency for Research on Cancer. We obtained biopsies from six control subjects and 10 patients with gastric carcinomas living in Manaus. In the patients, the samples were taken from tumors and from adjacent non-cancerous mucosa.
more » ... samples were screened for EBV DNA by PCR to amplify the 288 bp fragments from the Bam M region. P.F. de Aquino et al. The EBV DNA was detected in 8/10 of the tumor cases and in none of the six control subjects. In the positively identified samples, EBV DNA was detected in five corresponding resection margins. Previous research indicated only a weak association between EBV and gastric cancer. We suggest that EBV should be considered as a risk factor for gastric adenocarcinomas in Manaus.
doi:10.4238/2012.october.15.3 pmid:23096917 fatcat:4pzv7yr5djbjbko26z3bn43oou