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In vitro Plant Regeneration of Withania somnifera
2008
Plant Tissue Culture and Biotechnology
An efficient protocol was developed for large scale propagation using seed as an explant in Withania somnifera. MS supplemented with BAP at 0.6 mg/l with 0.4 mg/l IAA was found to be most effective in initiating multiple shoots at the rate of ten per explant. By repeated subculturing, a high frequency of shoot multiplication was established. About 90% rooting was achieved with 0.4 mg/l of IBA and 0.4 mg/l IAA. Micropropagated plants were hardened in half strength of MS and then established in (1 : 1) sand and soil mixture.
doi:10.3329/ptcb.v16i2.1112
fatcat:xpn66isyl5elzobiupv3ljgvia