In vitro Plant Regeneration of Withania somnifera

Ujjwala Supe, Fanisha Dhote, MG Roymon
2008 Plant Tissue Culture and Biotechnology  
An efficient protocol was developed for large scale propagation using seed as an explant in Withania somnifera. MS supplemented with BAP at 0.6 mg/l with 0.4 mg/l IAA was found to be most effective in initiating multiple shoots at the rate of ten per explant. By repeated subculturing, a high frequency of shoot multiplication was established. About 90% rooting was achieved with 0.4 mg/l of IBA and 0.4 mg/l IAA. Micropropagated plants were hardened in half strength of MS and then established in
more » ... en established in (1 : 1) sand and soil mixture.
doi:10.3329/ptcb.v16i2.1112 fatcat:xpn66isyl5elzobiupv3ljgvia