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The histologic evaluation of endochondral ossification is critical to the study of fracture healing, developmental biology, and comparative histology. A modification of Sayers' alcian blue hematoxylin staining technique is described in which a different counterstain is applied. Paraffin sections are stained in alcian blue hematoxylin for 30 min, and then placed in orange G-eosin counterstain for 1 min, 30 s. This method can differentiate cartilage, mature bone, and immature bone found indoi:10.1179/his.2008.31.1.19 fatcat:axtgg3co4bhwlk7xpgvsjvbltq