Maternal Co-ordinate Gene Regulation and Axis Polarity in the Scuttle Fly Megaselia abdita

Karl R. Wotton, Eva Jiménez-Guri, Johannes Jaeger, Claude Desplan
<span title="2015-03-10">2015</span> <i title="Public Library of Science (PLoS)"> <a target="_blank" rel="noopener" href="" style="color: black;">PLoS Genetics</a> </i> &nbsp;
Axis specification and segment determination in dipteran insects are an excellent model system for comparative analyses of gene network evolution. Antero-posterior polarity of the embryo is established through systems of maternal morphogen gradients. In Drosophila melanogaster, the anterior system acts through opposing gradients of Bicoid (Bcd) and Caudal (Cad), while the posterior system involves Nanos (Nos) and Hunchback (Hb) protein. These systems act redundantly. Both Bcd and Hb need to be
more &raquo; ... liminated to cause a complete loss of polarity resulting in mirror-duplicated abdomens, so-called bicaudal phenotypes. In contrast, knock-down of bcd alone is sufficient to induce double abdomens in nondrosophilid cyclorrhaphan dipterans such as the hoverfly Episyrphus balteatus or the scuttle fly Megaselia abdita. We investigate conserved and divergent aspects of axis specification in the cyclorrhaphan lineage through a detailed study of the establishment and regulatory effect of maternal gradients in M. abdita. Our results show that the function of the anterior maternal system is highly conserved in this species, despite the loss of maternal cad expression. In contrast, hb does not activate gap genes in this species. The absence of this activatory role provides a precise genetic explanation for the loss of polarity upon bcd knock-down in M. abdita, and suggests a general scenario in which the posterior maternal system is increasingly replaced by the anterior one during the evolution of the cyclorrhaphan dipteran lineage. Author Summary The basic head-to-tail polarity of an animal is established very early in development. In dipteran insects (flies, midges, and mosquitoes), polarity is established with the help of socalled morphogen gradients. Morphogens are regulatory proteins that are distributed as a concentration gradient, often involving diffusion from a localised source. This graded distribution then leads to the concentration-dependent activation of different target genes along the embryo's axis. We examine this process, which differs to a surprising extent between dipteran species, in the scuttle fly Megaselia abdita, and compare our results to the PLOS Genetics |
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="">doi:10.1371/journal.pgen.1005042</a> <a target="_blank" rel="external noopener" href="">pmid:25757102</a> <a target="_blank" rel="external noopener" href="">pmcid:PMC4355411</a> <a target="_blank" rel="external noopener" href="">fatcat:jsano5hlo5dr7is6tx4sttg6au</a> </span>
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