Fast and robust segmentation of spherical particles in volumetric data sets from brightfield microscopy

Olaf Ronneberger, Qing Wang, Hans Burkhardt
2008 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro  
In this article we present an approach for a precise segmentation of spherical particles in transmitted light image stacks. A main goal was its fast operation and a high robustness to occlusions and agglomerations of the particles. The system is based on a voting procedure that finds the centers and radii of the particles and a subsequent precise segmentation with an active contour approach. To meet the demands of an online pollenmonitor for high speed and low memory consumption a multi-scale
more » ... proach was applied. The proposed techniques successfully segmented the pollen grains in a vast amount of different air samples (about 2.7TB of raw data). The results on one of the most cluttered samples are presented in this paper.
doi:10.1109/isbi.2008.4541010 dblp:conf/isbi/RonnebergerWB08 fatcat:tu2myw3ufjbevogakwkrn2migu