The Anti-angiogenic Potential of a Phellodendron amurense Hot Water Extract in Vitro and ex Vivo
in Vitro와 ex vivo에서 황백 온수추출물의 신생혈관 억제효과

Eok-Cheon Kim, Seo Ho Kim, Kiho Bae, Han Sung Kim, Michael Gelinsky, Tack-Joong Kim
2015 Journal of Life Science  
Blocking new blood-vessel formation (angiogenesis) is now recognized as a useful approach to the therapeutic treatment of many solid tumors. The best validated approach to date is to target the vascular endothelial growth-factor (VEGF) pathway, a key regulator of angiogenesis. Many natural products and extracts that contain a variety of chemopreventive compounds have been shown to suppress the development of malignancies through their anti-angiogenic properties. Phellodendron amurense, which is
more » ... widely used in Korean traditional medicine, has been shown to possess antitumor, antimicrobial, and anti-inflammatory properties, among others. The present study investigated the effects of P. amurense hot-water extract (PAHWE) on angiogenesis, a key process in tumor growth, invasion, and metastasis. To investigate PAHWE's anti-angiogenic properties, this study's authors performed an analysis of angiogenesis and endothelial-cell proliferation, migration, invasion, and tube formation, as well as zymogram assays and the rat aortic ring-sprouting assay. PAHWE inhibited cell growth, mobility, and vessel formation in response to VEGF in vitro and ex vivo. Furthermore, it reduced VEGF-induced intracellular signaling events, such as the activation of matrix metalloproteinases (MMPs) -2 and -9. These results indicate that PAHWE's anti-angiogenic properties might lead to the development of potential drugs for treating angiogenesis-associated diseases such as cancer.
doi:10.5352/jls.2015.25.6.693 fatcat:vnb2i4jgkzbclkgmrrepne4tei