Inhibitory effects of tanshinone IIA from Salvia miltiorrhiza Bge on human bladder cancer BIU-87 cells and xenograft in nude mice
Food Science and Technology
The objective of this study was to investigate the inhibitory effects of tanshinone OOA on human bladder cancer BOU-87 cells and the xenograft in nude mice. BOU-87 cells were treated with tanshinone OOA with concentration of 0.5, 1, 2 and 4 g/L. The cell proliferation, cycle and apoptosis were detected. The nude mice with BOU-87 cell xenograft were treated with normal saline (control), 5-fluorouracil (5-FU), 200, 400 and 600 mg/kg tanshinone OOA, respectively. The animal body weight and growth
... weight and growth of tumor were measured. The expressions of B-cell lymphoma-2 (Bcl-2), Caspase-3 and proliferating cell nuclear antigen (PCNA) protein in xenograft were detected. The apoptosis index of cells was determined. Results showed that, tanshinone OOA inhibited the proliferation of BOU-87 cells, promoted their apoptosis, and arrested more cells in G 0 /G 1 phase. Tanshinone OOA significantly inhibited the growth of xenograft in nude mice and promoted the apoptosis of tumor cells in xenograft. Tanshinone OOA down-regulated Bcl-2 and PCNA expression and up-regulated Caspase-3 expression in xenograft. On conclusion, tanshinone OOA has inhibitory effects on human bladder cancer BOU-87 cells, and can inhibit the BOU-87 cells xenograft in nude mice. Practical Application: This study has provided a theoretical basis for clinical application of tanshinone OOA to treatment of bladder cancer.