Inhibitory effects of tanshinone IIA from Salvia miltiorrhiza Bge on human bladder cancer BIU-87 cells and xenograft in nude mice

Tao HUANG, Xiaokun YANG, Jianlei JI, Qinghai WANG, Hongyang WANG, Zhen DONG
2019 Food Science and Technology  
The objective of this study was to investigate the inhibitory effects of tanshinone OOA on human bladder cancer BOU-87 cells and the xenograft in nude mice. BOU-87 cells were treated with tanshinone OOA with concentration of 0.5, 1, 2 and 4 g/L. The cell proliferation, cycle and apoptosis were detected. The nude mice with BOU-87 cell xenograft were treated with normal saline (control), 5-fluorouracil (5-FU), 200, 400 and 600 mg/kg tanshinone OOA, respectively. The animal body weight and growth
more » ... weight and growth of tumor were measured. The expressions of B-cell lymphoma-2 (Bcl-2), Caspase-3 and proliferating cell nuclear antigen (PCNA) protein in xenograft were detected. The apoptosis index of cells was determined. Results showed that, tanshinone OOA inhibited the proliferation of BOU-87 cells, promoted their apoptosis, and arrested more cells in G 0 /G 1 phase. Tanshinone OOA significantly inhibited the growth of xenograft in nude mice and promoted the apoptosis of tumor cells in xenograft. Tanshinone OOA down-regulated Bcl-2 and PCNA expression and up-regulated Caspase-3 expression in xenograft. On conclusion, tanshinone OOA has inhibitory effects on human bladder cancer BOU-87 cells, and can inhibit the BOU-87 cells xenograft in nude mice. Practical Application: This study has provided a theoretical basis for clinical application of tanshinone OOA to treatment of bladder cancer.
doi:10.1590/fst.38818 fatcat:wf3w7gasqrakpmk7cntcsuigmi