Mass spectrometric identification of Rab23 phosphorylation as a response to challenge by cytidine 3′,5′-cyclic monophosphate in mouse brain

A. Elizabeth Bond, Edward Dudley, Robin Tuytten, Filip Lemière, Christopher J. Smith, Eddy L. Esmans, Russell P. Newton
2007 Rapid Communications in Mass Spectrometry  
While the functions and mechanisms of action of adenosine 3(,5(-cyclic monophosphate (cAMP) and guanosine 3(,5(-cyclic monophosphate (cGMP) are well established and are the basis of the action of a large number of successful pharmaceuticals, the role of a third naturally occurring cyclic nucleotide, cytidine 3(,5(-cyclic monophosphate (cCMP), remains to be elucidated. Immobilized metal affinity chromatography (IMAC) was used to selectively extract proteins phosphorylated in mouse brain in
more » ... se to challenge by cAMP, cGMP and cCMP, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToFMS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) of tryptic digests to identify Rab23 as the first protein reported to be phosphorylated only in response to cCMP.
doi:10.1002/rcm.3141 pmid:17639578 fatcat:p3gxekib3rgeph57fisrniaboy