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The Paralens™ (PL) microscope attachment converts a light microscope into an epi-fluorescence microscope. We compared the PL to standard fluorescence microscopy for detection of Mycobacteria in clinical and spiked samples. Overall agreement between the two systems was 100%. Quantitative and qualitative performance was comparable. The PL is an acceptable alternative to standard fluorescence microscopy for detection of Mycobacteria.doi:10.4236/jtr.2013.12004 fatcat:ltr2ci3yafaujkszmygdlobhse