Expression analysis of IL-15 as a candidate gene for placental efficiency in goats
Indian Journal of Animal Research
In present study, functional relevance of IL-15 with goat placenta was determined by quantitative real-time PCR (SYBR Green chemistry) and western blot. The 486 bp full-length coding sequence of the gene, encoding 162 amino acids, was cloned. Expression of IL-15 was significantly higher in placenta, especially in cotyledon, than that in other neonatal tissues (P less than 0.05). In both Dazu Black and Inner Mongolia Cashmere breeds, IL-15 mRNA in cotyledon was up-regulated at term compare to
... term compare to that at day 30 of gestation (P less than 0.05), and was significantly different between both breeds (P less than 0.05). Additionally, IL-15 transcripts had a close relationship with that of vascular endothelial growth factor and a positive correlation with placental efficiency (R2=0.922, P=0.04), but it was negatively correlated with cotyledon density (R2=0.952, P=0.024). The results indicate that IL-15 is conserved in goats, and has important role in placenta, thereby can be a candidate gene for placental efficiency in goats.