Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures

Angelo Luis Caron, Virgínia Picanço-Castro, Sven Ansorge, Dimas Tadeu Covas, Amine Kamen, Kamilla Swiech
2015 Brazilian Archives of Biology and Technology  
Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection
more » ... nt transfection with an inexpensive reagent, polyethylenimine (PEI) , reaching a total viral yield of 2.48x10 8 particles.
doi:10.1590/s1516-89132015060367 fatcat:brglnfphpnfqnpuusdnwnakt2u