Resonance Raman measurements of carotenoids using light-emitting diodes

Scott D. Bergeson, Justin B. Peatross, N. Jay Eyring, John F. Fralick, Douglas N. Stevenson, Scott B. Ferguson
2008 Journal of Biomedical Optics  
We report on the development of a compact commercial instrument for measuring carotenoids in skin tissue. The instrument uses two light emitting diodes (LEDs) for dual-wavelength excitation and four photomultiplier tubes for multichannel detection. Bandpass filters are used to select the excitation and detection wavelengths. The f/1.3 optical system has high optical throughput and single photon sensitivity, both of which are crucial in LED-based Raman measurements. We employ a signal processing
more » ... technique that compensates for detector drift and error. The sensitivity and reproducibility of the LED Raman instrument compares favorably to laser-based Raman spectrometers. This compact, portable instrument is used for non-invasive measurement of carotenoid molecules in human skin with a repeatability better than 10%.
doi:10.1117/1.2952075 pmid:19021353 fatcat:wyyvhfn3zzfxtieoal5aadx7ma