Aderência bacteriana e formação de biofilme aos fios de dermossustentação facial [thesis]

Bruna de Arruda Leite
LEITE, B.A. Bacterial adherence and biofilm formation on facial lifting threads. 2008. 121f. Dissertação (Mestrado em Bioengenharia). Programa de Pós-Graduação Interunidades em Bioengenharia -EESC/ FMRP/ IQSC da Universidade de São Paulo, São Carlos, 2008. Flabbiness and expression wrinkles can be helped by undergoing a face lifting or by implanting subcutaneous tissues of polyurethane or polypropylene threads. Under certain conditions microorganisms can attach themselves to the threads and
more » ... the threads and interact with these surfaces initiating cellular growth. The goal of the present study was to evaluate bacterial attachment to polyurethane and polypropylene threads by means of scanning electron microscopy and microbiological method. The threads were sectioned into segments of 1.0cm in length and inserted, one by one, into separated Falcon tubes (50 mL) containing Mueller Hinton broth (15 mL), with 200µl of the bacterial suspension (10 8 CFU/mL) prepared, and incubated for 1 hour and 30 minutes, at 4, 24, 48, 72 and 120 hour periods. After each incubation period, the coupons were rinsed three times and, inserted, one by one, into 5.0 mL of sterile physiological saline solution, sonicated at 40kHz for 8 minutes and vortexed for 10 seconds. This solution was diluted three-fold (1/10 to 1/1000), from dilution the 1/1000 dilution an aliquot of 0.1 mL was plated onto Tryptic Soy agar (TSA). The plates were incubated at 37ºC from 18 to 24h. After the incubation periods, the viable bacteria were counted and the results noted in terms of colony forming units (CFU/ml). The coupons destined for scanning electron microscopy observations were fixed in glutaraldehyde, dehydrated in alcohol series, dried in vacuum centryfuge and metalized with gold. A quantitative evaluation was recorded of the growth of S. aureus on the surface of polyurethane, after 1h and 30 minutes of contact, the results shown 4.0±0.0 CFU/ml, S. epidermidis 4.07±0.1 CFU/ml and P. aeruginosa 5.08±0.14 CFU/ml. After 4-120h the number of viable cells of S. aureus on polyurethane surfaces were 5.49±0,04 CFU/ml, S. epidermidis 4.99±0.07 CFU/ml and P. aeruginosa 6.52±0.03 CFU/ml. A quantitative evaluation was recorded of the growth of S. aureus on the surface of polypropylene after 1h and 30 minutes of contact, the results shown 4.24±0.20 CFU/ml, with S. epidermidis 4.14±0.1 CFU/ml and P. aeruginosa 5.77±0.05 CFU/ml. After 4-120h the number of viable cells of S. aureus on polypropylene surfaces were 5.96±0.07 CFU/ml, S. epidermidis 4.96±0.07 CFU/ml and P. aeruginosa 6.63±0.05 CFU/ml. The number of viable cells of S. aureus, S. epidermidis, P. aeruginosa on polyurethane and polypropylene surfaces were significantly different (p<0.05). A biofilm was observed on both threads (polyurethane and polypropylene) as demonstrated by scanning electron microscopy. KeyWords: Threads of lifitng. Polyurethane. Polypropylene. Biofilm. Bacterial adherence. IGNÁCIO,H.; MAZZER,N.; BARBIERI, C.H.; CHIERICE,G.O. Utilização da poliuretana de mamona nas formas compacta e porosa no preenchimento de falha óssea: estudo experimental em cães. Rev. Bras. Ortop., v.37, n.5, p.187-194, 2002. JI, J.; ZHANG, W. Bacterial behaviors on polymer surface with organic and inorganic antimicrobial compounds. J. Biomed. Materials Res., 2008. JONES, J.W.; JURKOVICH, G.P. Polypropylene mesh closure of infected abdominal wounds. Am. Surg., v.55, p.73-6, 1999. JOYANES, P.; PASCUAL, A.; MARTÍNEZ-MARTÍNEZ, L.; HEVIA, A.; PEREA, E.J. In Vitro Adherence of Enterococcus faecalis and Enterococcus faecium to Urinary Catheters. J. Microbiol. Infects Dis., v.19, p.124-127, 2000. KATSIKOGIANNI, M.; SPILIOPOULOU, I.; DOWLING, D.P.; MISSIRLIS, Y,F. Adhesion of slime producing Staphylococcus epidermidis strains to PVC and diamont-like carbon/silver/fluorinated coatings. Mater. Sci. Mater. Med., v.8, 2006. KHARDORI, N.; YASSIEN, M.L. Biofilms in device-related infections. J. Microbiol., v.15, p.141-417, 1995. KUMON, H.K; TOMOCHIKA, K.; MATUNAGA, T.; OGAWA M.; OHMORI, H. A sandwich cup method for the penetration assay of antimicrobial agents through Pseudomonas aeruginosa expolysaccharides. Microbiol. Immunol., v.38, p.615-619, 1994. LAW, N.W.; ELLIS, H. A comparison of polypropylene mesh and expanded polytetrafluorrthylene patch for the repair of contamined abdominal wall defects. An experimental study Surgery., v.109, p.652-9, 1991. LAWRENCE, J.R.; KORBER, D.R.; HOYLE, B.D.; COSTERTON, J.W.; CALDWELL, D.E. Optical sectioning of microbial biofilms. SOBRINHO, J.A.; RAMALHO, L.T.O.; MANGILLI, P.D.; RAPOPORT, A. The action of the castor bean polymer during the bone neoformation. Acta. Cir. Bras., v.19, n.4, 2004. LI, X.; KING, M.; MACDONALD, P. Comparative study of knot performance and ease of manipulation of monofilament and braided sutures for arthroscopic applications. Rev. Traumatol. Arthrosc., v.12, p.448-52, 2004. LOCATELLI, C.I.; ENGLERT, G.E.; KWITKO, S.; SIMONETTI, A.B. In vitro bacterial adherence to silicone and polymetylmetacrylate intraocular lenses. Arq. Bras. Oftalmol., v.67, n.2, 2004. LOPEZ-LOPEZ, G.; PASCUAL, A.; PEREA, E.J. Effect of plastic catheter material on bacterial adherence and viability.
doi:10.11606/d.82.2008.tde-16102008-115949 fatcat:ss7aj5pfvbdlhl4wbliwg6alg4