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TCR analysis reveals significant repertoire selection during in vitro lymphocyte culture
The in vitro stimulation of T lymphocytes is frequently used as a technique to expand specific cells present at low precursor frequency in vivo. However, cells analysed after such procedures may no longer reflect those originally present in vivo because of the variable efficiency of outgrowth of different T cell subpopulations. To systematically assess this and to complement functional assays, we have analysed the TCR repertoire using a new high resolution RT-PCR method to determine TCR β chaindoi:10.1093/intimm/9.8.1073 pmid:9263004 fatcat:x6ixdemwlbcy5h45sexksfqbyq