Analysis of rivastigmine in in vitro transdermal permeation studies by RP-HPLC-PDA method

Shaik Firdose, P Prashanth, V Srikalyani, S Madhavi, Buchi Nalluri
2013 Journal of Chemical and Pharmaceutical Research   unpublished
The aim of the present work is to develop and validate a simple, efficient, economical RP-HPLC method for the analysis of Rivastigmine (RVS) in drug in adhesive type transdermal patches and in transdermal permeation and/or release studies. An Inertsil-ODS-3 column (250 x 4.6mm, 5µm) with mobile phase containing 10mM ammonium acetate: methanol (35:65% v/v) at a flow rate of 1.0mL/min was used in isocratic mode and eluents were monitored at 217nm. The retention time of RVS was 5.32 min and showed
more » ... 5.32 min and showed a good linearity in the concentration range of 2-10µg/mL with a correlation coefficient >0.999. The validation parameters like specificity, linearity, accuracy and limit of detection, limit of quantification, precision, robustness fulfilled regulatory requirements. The percent recoveries were ranged in between 98-102, (RSD < 2). The developed HPLC method was successfully used for the analysis of RVS in bulk and in house made transdermal patches and in transdermal permeation and/or release studies.
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