A line scanned light-sheet microscope with phase shaped self-reconstructing beams

Florian O. Fahrbach, Alexander Rohrbach
2010 Optics Express  
We recently demonstrated that Microscopy with Self-Reconstructing Beams (MISERB) increases both image quality and penetration depth of illumination beams in strongly scattering media. Based on the concept of line scanned light-sheet microscopy, we present an add-on module to a standard inverted microscope using a scanned beam that is shaped in phase and amplitude by a spatial light modulator. We explain technical details of the setup as well as of the holograms for the creation, positioning and
more » ... on, positioning and scaling of static light-sheets, Gaussian beams and Bessel beams. The comparison of images from identical sample areas illuminated by different beams allows a precise assessment of the interconnection between beam shape and image quality. The superior propagation ability of Bessel beams through inhomogeneous media is demonstrated by measurements on various scattering media.
doi:10.1364/oe.18.024229 pmid:21164769 fatcat:g7yw2dp5oje7nioqcczqk4maym