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Blooms of the microalga Prymnesium parvum cause devastating fish kills worldwide, which are suspected to be caused by the supersized ladder-frame polyether toxins prymnesin-1 and -2. These toxins have, however, only been detected from P. parvum in rare cases since they were originally described two decades ago. Here, we report the isolation and characterization of a novel B-type prymnesin, based on extensive analysis of 2D-and 3D-NMR data of natural as well as 90% 13 C enriched material. B-typedoi:10.1021/acs.jnatprod.6b00345.s001 fatcat:7hbwbvrtvvhyxazw6vdstlisc4