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A Fluorogenic Array Tag for Temporally Unlimited Single Molecule Tracking
[article]
2017
bioRxiv
pre-print
Cellular processes take place over many timescales, prompting the development of precision measurement technologies that cover milliseconds to hours. Here we describe ArrayG, a bipartite fluorogenic system composed of a GFP-nanobody array and monomeric wtGFP binders. The free binders are initially dim but brighten 15 fold upon binding the array, suppressing background fluorescence. By balancing rates of intracellular binder production, photo-bleaching, and stochastic binder exchange on the
doi:10.1101/159004
fatcat:ocid62j3n5dmfkfkaeq5kddyi4