Fifty-one liver tissue biopsies of full-term stillbirths from HIV-mono-infected mothers were investigated. All morphometric indices of non-parenchymal liver cells were calculated using the Avtandilov microscopic morphometric grid, which consisted of 100 equidistant points. It was inserted into the microscope ocular tube with a total × 200 microscope magnification. The number of points that were found in the corresponding types of non-parenchymal liver cells determined by immunohistochemistry

more »

... calculated. In each case, 10 random microscopic areas were selected and then all data were obtained, calculated and presented as percentages. Morphometric indices of non-parenchymal liver cells: Kupffer cells - 28.3±2.9% [control - 21.2 ±2.5], hepatic stellate cells - 14.1 ±1.4% [control - 9.8 ±1.2], LAL (liver-associated lymphocytes): CD4 - 29.2 ±2.2% [control - 43.3 ±4.9], CD8 - 25.3 ±1.9% [control - 23.1 ±1.7], dendritic cells - 3.9 ±0.3% [control - 2.6 ±0.3]. It was established that at the time of the birth of children from HIV-mono-infected mothers the Kupffer cells showed signs of their proliferation and liver-associated CD4 lymphocytes showed signs of their reduction; hepatic stellate cells showed signs of their proliferation and hyperactivation; dendritic liver cells showed signs of their proliferation.