Isomerization of an enzyme-coenzyme complex in yeast alcohol dehydrogenase-catalysed reactions

Vladimir Leskovac, Svetlana Trivic, Draginja Pericin
2003 Journal of the Serbian Chemical Society  
In this work, all the rate constants in the kinetic mechanism of the yeast alcohol dehydrogenase-catalyzed oxidation of ethanol by NAD + , at pH 7.0, 25 ºC, have been estimated. The determination of the individual rate constants was achieved by fitting the reaction progress curves to the experimental data, using the procedures of the FITSIM and KINSIM software package of Carl Frieden. This work is the first report in the literature showing the internal equilibrium constants for the
more » ... or the isomerization of the enzyme-NAD + complex in yeast alcohol dehydrogenase-catalyzed reactions.
doi:10.2298/jsc0302077l fatcat:cl4ecm7tpbdotk5ouhbav6gdie